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肺泡上皮组织是通过分化原代肺泡上皮细胞在细胞培养液中产生的, 肺内皮细胞和成纤维细胞使用一系列专有的培养基. 分化的肺泡上皮组织有一个鳞状上皮，由I型和II型肺泡上皮细胞组成，细胞外基质培养产生肺成纤维细胞和内皮细胞. 上皮具有紧密的功能连接和强大的屏障，在30天以上的研究中保持稳定.
肺泡上皮细胞发展的一部分资金是由 PETA International Science Consortium.
肺泡表面三维人体组织模型通常用于包括安全在内的一系列应用 & risk assessment, inflammation & fibrosis, and drug delivery. 简单的协议和对早期细胞终点的评估使科学家能够在几天内获取数据, not weeks or months.
Inhalation Toxicology
使用肺泡上皮来确定吸入物质的相对安全性和毒理学潜力.
Disease Modeling
Hana Barosova, Anna G. Maione, Dedy Septiadi, Monita Sharma, Laetitia Haeni, Sandor Balog, Olivia O’Connell, George R. Jackson, David Brown, Amy J. Clippinger, Patrick Hayden, Alke Petri-Fink, Vicki Stone, and Barbara Rothen-Rutishauser. ACS Nano Article ASAP. DOI: 10.1021/acsnano.9b06860
Drug Development
Host-Pathogen Interaction
Tissue
Kit: 一个标准的肺泡上皮试剂盒(alv -100 ft - pe12)由24个组织组成. (Tissue “kits” contain tissues, 250 mL of culture medium, and plasticware; contact 华体会网页版 for specific kit contents). ALV-100-FT-MAC-PE12，含有THP-1巨噬细胞.
Formats: 12mm Transwell插入物-组织培养基质经化学改性，孔径为0.4 μm
Culture: Air-liquid interface
Histology: 1-2层细胞-高度鳞状形态和组织结构.
Lot numbers: Tissue lots produced each week are assigned a specific lot number. 一个字母被附加在批号的末尾，以区分在给定的许多组织中的单个试剂盒. All tissue kits within a lot are identical in regards to cells, medium, handling, culture conditions, etc.
Shipment: At 4°C on medium-supplemented, agarose gels
Shipment Day: ALV-100-FT-PE12 – Every Monday. ALV-100-FT-MAC-PE12 – Every Tuesday.
Delivery: ALV-100-FT-PE12 – Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Wednesday depending on location. alv -100 ft - mac - pe12 -周三上午通过联邦快递优先服务(美国). Outside US: Wednesday-Thursday depending on location.
Shelf Life: 包括运输时间在内，组织可在使用前在4°C保存3天. However, extended storage periods are not recommended. In addition, 如果组织在同一天连续使用，可获得最好的重现性.
Length of Experiments: 组织培养可持续2周或更长时间，保持良好的正常形态. Tissues must be fed every other day with 5.0 ml of maintenance medium (ALV-100-MM). 细胞培养片置于提供的6孔悬挂培养板中(HNG-TOP-6-22).
Cells
Type: Normal human alveolar epithelial cells (NHAE); Normal human pulmonary fibroblasts (NHPF), 正常人肺内皮细胞(NHPE)和THP-1巨噬细胞(ALV-100-FT-MAC-PE12).
Genetic make-up: Multiple donors
Derived from: Healthy, non-smoker
Screened for: HIV, Hepatitis-B, Hepatitis-C, mycoplasma
Medium
Base medium: Proprietary
Growth factors/hormones: Epidermal growth factors and other proprietary factors
Antibiotics: Gentamicin 30 µg/ml
Anti-fungal agent: Amphotericin B 15 ng/ml
pH Indicator: Phenol red
Other additives: Proprietary
Maintenance medium: ALV-100-MM
Quality Control and Sterility
Visual inspection: 所有组织都要进行目视检查，如果发现了物理缺陷, tissues are rejected for shipment
End-use testing: 使用EVOM2上皮细胞伏安计和Endohm电极室(世界精密仪器)测量每个肺泡表面批的经上皮细胞电阻(TEER).
Sterility: 在整个生产过程中使用的所有培养基都要进行无菌检查. 维持培养基不加抗生素孵育1周，检查无菌情况. 来自24孔板的用于运输的琼脂糖凝胶也被培养1周，并检查任何污染的迹象
Screening for pathogens: 对所有细胞进行筛选，并使用PCR检测艾滋病毒、乙型肝炎和丙型肝炎均为阴性. 然而，没有任何已知的测试方法可以完全保证这些细胞是无病原体的. Thus, 这些产品和所有人类源性产品应按照CDC-NIH手册建议的BSL-2级别(生物安全级别2)或更高级别进行处理, “Biosafety in microbiological and biomedical laboratories,” 1998. 如需进一步协助，请联系您的现场安全主任或华体会网页版技术服务
Notification of lot failure: If a tissue lot fails our QC or sterility testing, 顾客会得到通知，并在适当的时候免费更换纸巾. Because our QC and sterility testing is done post-shipment, 会尽快发出通知(在正常情况下, TEER failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)
