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华体会网页版’s Epi角膜 tissue model consists of normal human corneal epithelial cells cultured to form a stratified, squamous epithelium which closely parallels normal human corneal tissue. 角膜细胞, cultured on specially prepared cell culture inserts with serum-free Medium, 分层形成多层次结构.
用于药物传递和药物开发, Epi角膜 is a mitotically and metabolically active tissue with barrier properties approaching those of the human cornea. Epi角膜 can be stimulated to release many cytokines known to be important in inflammation and diseases such as dry eye. 进一步, the advanced 3D tissue model expresses cornea specific drug transporters and metabolizing enzymes.
角膜给药与角膜毒性
外膜严密地概括了生理机能, 3 d组织架构, and function of the human cornea for use in pharmaceutical development applications.
博士联系. 尤利娅•Kaluzhny 的更多信息.
Latanoprost药物输送
模型复方给药
干眼病
角膜伤口愈合
浏览我们的 参考图书馆 to see how our Epi角膜 tissue has been used in these areas of study.
组织
工具包: A standard Epi角膜 kit (COR-100) consists of 24 tissues. (组织“套件”包含组织, 少量培养基:少量培养基, and plasticware; contact 华体会网页版 for specific kit contents)
格式: 9mm individual inserts – tissue culture substrate is chemically modified with a pore size of 0.4 μm
文化:气液界面
组织学: 4-5 cell layers – stratified, squamous morphology and tissue structure
很多数字: 组织 lots produced each week are assigned a specific lot number. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. All tissue kits within a lot are identical in regards to cells, Medium, 处理, 培养条件, 等.
装运:在4°C中补充琼脂糖凝胶
装船日:每一个星期一
交付:星期二上午，通过联邦快递优先服务(美国). 美国以外:周二至周四，取决于地点
保质期: Including time in transit, tissues may be stored at 4°C for up to 3 days prior to use. 然而, extended storage periods are not recommended unless necessary. 除了, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. Tuesday afternoon or following overnight storage at 4°C (Wednesday morning)
细胞
类型:正常人角膜上皮细胞(HCEC)
基因组成:单一捐赠者
来自:健康的捐赠者
筛查: HIV，乙型肝炎，丙型肝炎，支原体
Medium
基础培养基: 杜尔贝科改良鹰型介质(DMEM).
生长因子/激素: 表皮生长因子, 胰岛素, hydrocortisone and other proprietary stimulators of corneal epithelial differentiation.
血清: 没有一个.
抗生素: 青霉素(50 μ g/ml) /链霉素(50µg/ml)
抗真菌剂: 两性霉素B (0.125µg / ml).
酸碱指示剂: 酚红.
选择: 酚红-free, antibiotic/anti-fungal-free, or hydrocortisone-free Medium and tissue are available. 代理商至少在装运前3天被移除.
维护中: Most experiments with Epi角膜™ are performed within 48 hours. 然而, 长时间的实验, the tissue can be maintained in COR-100-MM (identical to Epi角膜™ assay Medium).
质量控制和无菌
目视检查: All tissues are visually inspected and if physical imperfections are noted, 组织被拒绝装运
不育: All media used throughout the production process is checked for sterility. Maintenance Medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination
筛查病原体: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. 然而, no known test method can offer complete assurance that the cells are pathogen free. 因此, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, "微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or 华体会网页版 technical service
批错通知书:如果组织批没有通过我们的质量控制或无菌测试, the customer will be notified and the tissues will be replaced without charge when appropriate. 因为我们的QC和无菌检测是在出货后进行的, notification will be made as soon as possible (Under normal circumstances, ET-50故障将在周三下午5点前通知.m.; sterility failures will be notified within 8 days of shipment)
